The concept of leadership has been highly evaluated and investigated in Western literature. Whether the concept of leadership and leadership behaviors adopted from the West will be perceived with the same rigidity in Thailand settings requires academic investigation. Moreover, the investigation must be extended beyond the physical world and into the virtual realm given globalization in the Internet age. Advancement of modern communication technology has enabled interaction and cooperation among individuals worldwide. The interactivity is observable as virtual teaming and also exists in professional gameplay known as eSports. In contrast to face-to-face teaming, eSports has overcome the limitations of geographical location and national boundaries by having all players follow the same rules and goals. Accordingly, the concept of leadership may underlie interactions within the virtual environment and should be examined for its universality. Using an exploratory research design, this study explored the applicability of real-world leadership behaviors and virtual leadership through eSports. The results of a principle components analysis (PCA) led to the conclusion that both real- and virtual-world leadership behaviors are applicable to Thai eSports players.